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Published Online: 17 July 2015

Detection of Circulating miRNA Levels in Schizophrenia

Hui Wei, Ph.D., Yanbo Yuan, M.D., Sha Liu, Ph.D., Cheng Wang, Ph.D., Fude Yang, M.D., Zheng Lu, M.D., Chuanyue Wang, M.D., Hong Deng, M.D., Jinping Zhao, M.D., Yan Shen, M.S., Chenyu Zhang, Ph.D., Xin Yu, M.D., and Qi Xu, M.D., Ph.D.Authors Info & Affiliations
Publication: American Journal of Psychiatry
Volume 172, Number 11
https://doi.org/10.1176/appi.ajp.2015.14030273
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Abstract

Objective:

Diagnosis of schizophrenia is currently dependent on symptom-based criteria and lacks objective indicators. In this study, the authors investigated whether circulating miRNA can serve as a diagnostic biomarker for schizophrenia.

Methods:

Global plasma miRNAs were profiled in a test cohort of 164 schizophrenia patients and 187 control subjects, using Solexa sequencing, TaqMan Low Density Array, and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays. The captured miRNAs were then validated by qRT-PCR assays in an independent cohort of 400 schizophrenia patients, 213 control subjects, and 162 patients with nonschizophrenia psychiatric disorders; the 400 schizophrenia patients underwent a 12-month follow up study of regular treatment with an atypical antipsychotic (risperidone and aripiprazole).

Results:

The global plasma miRNA screening revealed eight miRNAs that were up-regulated in schizophrenia, as revealed by both assay platforms. The qRT-PCR analysis showed the up-regulation of miR-130b and miR-193a-3p in schizophrenia but not in nonschizophrenia disorders.

Conclusions:

The up-regulation of miR-130b and miR-193a-3p is a state-independent biomarker for schizophrenia, and these two miRNAs could be used to develop a diagnostic tool for schizophrenia.

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Supplementary Material

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Information & Authors

Information

Published In

Go to American Journal of Psychiatry
Go to American Journal of Psychiatry
American Journal of Psychiatry
Volume 172 • Number 11 • November 01, 2015
Pages: 1141 - 1147
PubMed: 26183697

History

Received: 3 March 2014
Revision received: 28 October 2014
Revision received: 6 January 2015
Revision received: 14 March 2015
Accepted: 3 April 2015
Published online: 17 July 2015
Published in print: November 01, 2015

Authors

Affiliations

Hui Wei, Ph.D.
From the National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences and Neuroscience Center, Chinese Academy of Medical Science and Peking Union Medical College, Beijing; Peking University Institute of Mental Health, Peking University Sixth Hospital, Key Laboratory of Mental Health, Ministry of Health (Peking University), Beijing; Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing; Center for Biological Psychiatry, Beijing Hui-Long-Guan Hospital, Beijing; Shanghai Mental Health Center, Shanghai; Beijing Anding Hospital, Beijing; Mental Health Center, West China Hospital, Sichuan University, Chengdu; Mental Health Institute, Second Xiangya Hospital, Central South University, Changsha.
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Yanbo Yuan, M.D.
From the National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences and Neuroscience Center, Chinese Academy of Medical Science and Peking Union Medical College, Beijing; Peking University Institute of Mental Health, Peking University Sixth Hospital, Key Laboratory of Mental Health, Ministry of Health (Peking University), Beijing; Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing; Center for Biological Psychiatry, Beijing Hui-Long-Guan Hospital, Beijing; Shanghai Mental Health Center, Shanghai; Beijing Anding Hospital, Beijing; Mental Health Center, West China Hospital, Sichuan University, Chengdu; Mental Health Institute, Second Xiangya Hospital, Central South University, Changsha.
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Sha Liu, Ph.D.
From the National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences and Neuroscience Center, Chinese Academy of Medical Science and Peking Union Medical College, Beijing; Peking University Institute of Mental Health, Peking University Sixth Hospital, Key Laboratory of Mental Health, Ministry of Health (Peking University), Beijing; Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing; Center for Biological Psychiatry, Beijing Hui-Long-Guan Hospital, Beijing; Shanghai Mental Health Center, Shanghai; Beijing Anding Hospital, Beijing; Mental Health Center, West China Hospital, Sichuan University, Chengdu; Mental Health Institute, Second Xiangya Hospital, Central South University, Changsha.
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Cheng Wang, Ph.D.
From the National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences and Neuroscience Center, Chinese Academy of Medical Science and Peking Union Medical College, Beijing; Peking University Institute of Mental Health, Peking University Sixth Hospital, Key Laboratory of Mental Health, Ministry of Health (Peking University), Beijing; Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing; Center for Biological Psychiatry, Beijing Hui-Long-Guan Hospital, Beijing; Shanghai Mental Health Center, Shanghai; Beijing Anding Hospital, Beijing; Mental Health Center, West China Hospital, Sichuan University, Chengdu; Mental Health Institute, Second Xiangya Hospital, Central South University, Changsha.
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Fude Yang, M.D.
From the National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences and Neuroscience Center, Chinese Academy of Medical Science and Peking Union Medical College, Beijing; Peking University Institute of Mental Health, Peking University Sixth Hospital, Key Laboratory of Mental Health, Ministry of Health (Peking University), Beijing; Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing; Center for Biological Psychiatry, Beijing Hui-Long-Guan Hospital, Beijing; Shanghai Mental Health Center, Shanghai; Beijing Anding Hospital, Beijing; Mental Health Center, West China Hospital, Sichuan University, Chengdu; Mental Health Institute, Second Xiangya Hospital, Central South University, Changsha.
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Zheng Lu, M.D.
From the National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences and Neuroscience Center, Chinese Academy of Medical Science and Peking Union Medical College, Beijing; Peking University Institute of Mental Health, Peking University Sixth Hospital, Key Laboratory of Mental Health, Ministry of Health (Peking University), Beijing; Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing; Center for Biological Psychiatry, Beijing Hui-Long-Guan Hospital, Beijing; Shanghai Mental Health Center, Shanghai; Beijing Anding Hospital, Beijing; Mental Health Center, West China Hospital, Sichuan University, Chengdu; Mental Health Institute, Second Xiangya Hospital, Central South University, Changsha.
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Chuanyue Wang, M.D.
From the National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences and Neuroscience Center, Chinese Academy of Medical Science and Peking Union Medical College, Beijing; Peking University Institute of Mental Health, Peking University Sixth Hospital, Key Laboratory of Mental Health, Ministry of Health (Peking University), Beijing; Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing; Center for Biological Psychiatry, Beijing Hui-Long-Guan Hospital, Beijing; Shanghai Mental Health Center, Shanghai; Beijing Anding Hospital, Beijing; Mental Health Center, West China Hospital, Sichuan University, Chengdu; Mental Health Institute, Second Xiangya Hospital, Central South University, Changsha.
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Hong Deng, M.D.
From the National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences and Neuroscience Center, Chinese Academy of Medical Science and Peking Union Medical College, Beijing; Peking University Institute of Mental Health, Peking University Sixth Hospital, Key Laboratory of Mental Health, Ministry of Health (Peking University), Beijing; Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing; Center for Biological Psychiatry, Beijing Hui-Long-Guan Hospital, Beijing; Shanghai Mental Health Center, Shanghai; Beijing Anding Hospital, Beijing; Mental Health Center, West China Hospital, Sichuan University, Chengdu; Mental Health Institute, Second Xiangya Hospital, Central South University, Changsha.
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Jinping Zhao, M.D.
From the National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences and Neuroscience Center, Chinese Academy of Medical Science and Peking Union Medical College, Beijing; Peking University Institute of Mental Health, Peking University Sixth Hospital, Key Laboratory of Mental Health, Ministry of Health (Peking University), Beijing; Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing; Center for Biological Psychiatry, Beijing Hui-Long-Guan Hospital, Beijing; Shanghai Mental Health Center, Shanghai; Beijing Anding Hospital, Beijing; Mental Health Center, West China Hospital, Sichuan University, Chengdu; Mental Health Institute, Second Xiangya Hospital, Central South University, Changsha.
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Yan Shen, M.S.
From the National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences and Neuroscience Center, Chinese Academy of Medical Science and Peking Union Medical College, Beijing; Peking University Institute of Mental Health, Peking University Sixth Hospital, Key Laboratory of Mental Health, Ministry of Health (Peking University), Beijing; Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing; Center for Biological Psychiatry, Beijing Hui-Long-Guan Hospital, Beijing; Shanghai Mental Health Center, Shanghai; Beijing Anding Hospital, Beijing; Mental Health Center, West China Hospital, Sichuan University, Chengdu; Mental Health Institute, Second Xiangya Hospital, Central South University, Changsha.
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Chenyu Zhang, Ph.D.
From the National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences and Neuroscience Center, Chinese Academy of Medical Science and Peking Union Medical College, Beijing; Peking University Institute of Mental Health, Peking University Sixth Hospital, Key Laboratory of Mental Health, Ministry of Health (Peking University), Beijing; Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing; Center for Biological Psychiatry, Beijing Hui-Long-Guan Hospital, Beijing; Shanghai Mental Health Center, Shanghai; Beijing Anding Hospital, Beijing; Mental Health Center, West China Hospital, Sichuan University, Chengdu; Mental Health Institute, Second Xiangya Hospital, Central South University, Changsha.
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Xin Yu, M.D.
From the National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences and Neuroscience Center, Chinese Academy of Medical Science and Peking Union Medical College, Beijing; Peking University Institute of Mental Health, Peking University Sixth Hospital, Key Laboratory of Mental Health, Ministry of Health (Peking University), Beijing; Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing; Center for Biological Psychiatry, Beijing Hui-Long-Guan Hospital, Beijing; Shanghai Mental Health Center, Shanghai; Beijing Anding Hospital, Beijing; Mental Health Center, West China Hospital, Sichuan University, Chengdu; Mental Health Institute, Second Xiangya Hospital, Central South University, Changsha.
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Qi Xu, M.D., Ph.D.
From the National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences and Neuroscience Center, Chinese Academy of Medical Science and Peking Union Medical College, Beijing; Peking University Institute of Mental Health, Peking University Sixth Hospital, Key Laboratory of Mental Health, Ministry of Health (Peking University), Beijing; Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing; Center for Biological Psychiatry, Beijing Hui-Long-Guan Hospital, Beijing; Shanghai Mental Health Center, Shanghai; Beijing Anding Hospital, Beijing; Mental Health Center, West China Hospital, Sichuan University, Chengdu; Mental Health Institute, Second Xiangya Hospital, Central South University, Changsha.
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Notes

Address correspondence to Dr. Xu ([email protected]), Dr. Yu ([email protected]), and Dr. Zhang ([email protected]).

Author Contributions

The first two authors contributed equally to this work.

Competing Interests

The authors report no financial relationships with commercial interests.

Funding Information

Supported by the National 973 Projects (2013CB531300, 2012CB517902, 2014CB542300, 201302018), National Key Project of Scientific and Technical Supporting Programs funded by MOST of China (2007BAI17B04), the National Natural Science Foundation of China (31222031, 31430048, 81471325), the Fundamental Research Funds for the Central Universities (2012S05), and the Peking Union Medical College Youth Fund (2012J09, NCET-12-0071).

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