Neuroactive Steroids and Suicidality in Posttraumatic Stress Disorder
Publication: American Journal of Psychiatry
Abstract
OBJECTIVE: Recent studies suggest that neuroactive steroids may be altered in posttraumatic stress disorder (PTSD). Since high rates of suicidality accompany PTSD, the authors investigated neuroactive steroid levels and correlations to suicide attempts in veterans with this disorder. METHOD: Male veterans with PTSD enrolled in a larger study during inpatient hospitalization (N=130) were assessed for suicidal ideation or suicide attempt in the last 6 months. Serum levels of dehydroepiandrosterone (DHEA), androstenedione, testosterone, and estradiol were determined. The authors investigated associations between neuroactive steroids and suicidality. RESULTS: High rates of suicidality were observed. Close to 70% of these patients had suicidal thoughts, and 25% had attempted suicide in the last 6 months. Patients who had attempted suicide demonstrated significantly higher median DHEA levels than those who had not attempted suicide (15.6 versus 8.3 ng/ml), an association that persisted after adjustment for age. CONCLUSIONS: These findings suggest that higher DHEA levels may be linked to suicidality in veterans with PTSD and may be associated with the risk of self-harm.
Patients with posttraumatic stress disorder (PTSD) have high rates of suicidality (1–3), and comorbid PTSD increases suicidal behavior risk in depression (4). There is also evidence of neuroendocrine alterations in PTSD (5, 6), including elevations in the neuroactive steroid dehydroepiandrosterone (DHEA) and its sulfated derivative (7, 8). Neuroactive steroids rapidly alter neuronal excitability by acting at ligand-gated ion channel receptors in the cell membrane such as γ-aminobutyric acid type A (GABAA) receptors (9), among others. In addition to peripheral synthesis in the adrenals and gonads, many neuroactive steroids are synthesized de novo in the brain from cholesterol (neurosteroids). They are increased during the acute stress response (10, 11) and may therefore modulate the hypothalamic-pituitary-adrenal changes observed in PTSD. For example, the neuroactive steroid DHEA has antiglucocorticoid effects (12, 13). It is also anxiolytic in rodent behavioral models (14), but it negatively modulates GABAA receptors (15, 16). Since DHEA administration significantly elevates plasma levels of the neuroactive steroid allopregnanolone (17), it is possible that DHEA-induced increases in this positive modulator of GABAA receptors contribute to DHEA anxiolytic effects. These data suggest that neuroactive steroids may be relevant to the pathophysiology of PTSD and other anxiety disorders. Little is known regarding possible associations between neuroactive steroids and suicidal behaviors in PTSD. Therefore, we hypothesized that neuroactive steroid alterations may be related to suicidality in PTSD and investigated neuroactive steroid levels in male veterans with this disorder.
Method
We tested serum from 130 male veterans meeting DSM-IV criteria for PTSD who were enrolled in a larger multisite study of HIV seroprevalence and risks from the Durham Veterans Affairs inpatient psychiatric unit between March 1997 and June 2000. PTSD diagnosis was based on archival record review, in addition to the following criteria: an exacerbation of PTSD symptoms prompting the current psychiatric admission or military-service-connection status for PTSD at the time of admission. The PTSD Checklist was used for diagnostic confirmation (mean score=67, consistent with severe PTSD) (18). Patients were evaluated with a validated structured risk interview composed of standardized measures that included the following domains: suicidal ideation or suicide attempt in the 6 months prior to admission, demographic information (age, ethnicity, marital status, educational level), smoking status and alcohol use disorders (Dartmouth Attitudes and Lifestyle Inventory Scale), and childhood sexual (Sexual Abuse Exposure Questionnaire) and physical (Conflict Tactics Scale) trauma (19). All study participants provided written informed consent for the risk interview and serum sampling in the larger study. Samples were deidentified for the steroid assays, and the protocol was approved by the local institutional review board.
Serum was collected from each patient at 6:30 a.m. within 3 days of inpatient psychiatric hospitalization. Radioimmunoassay analyses were performed (Diagnostic Products Corp., Los Angeles; ICN Pharmaceuticals, Inc., Costa Mesa, Calif.) for a total of four steroids across a biosynthetic pathway: DHEA → androstenedione → testosterone (total) → estradiol. Intra- and interassay coefficients of variation were less than 9% for all four radioimmunoassays, and cross-reactivity with other steroids was minimal. Neuroactive steroid levels were obtained for most subjects; missing data secondary to limited serum volume were addressed by using case-wise deletion.
Wilcoxon rank sum statistics were used to examine bivariate associations between neuroactive steroids and suicidality. Bivariate associations between suicidality and smoking status, alcohol use disorder, and childhood trauma were examined by using Pearson chi-square statistics. Separate logistic regression models were examined for each neuroactive steroid, including age as a covariate (SAS version 8.2, SAS Institute, Cary, N.C.).
Results
A total of 130 male veterans with PTSD were evaluated; their mean age was 49.35 years (SD=8.13). This patient group was predominantly African American (N=62 [48%]) or Caucasian (N=63 [48%]), approximately one-third were married (N=48 [37%]), and more than half had been educated beyond high school (N=81 [62%]). Almost two-thirds (N=83 [64%]) had concurrent tobacco use, and 57 (44%) had alcohol use disorder. The majority had combat exposure (N=122 [94%]) and a history of childhood physical and/or sexual abuse (N=94 [72%]).
Our primary outcome variable was a suicide attempt in the last 6 months by patient self-report. One-quarter (N=33 [25%]) reported a suicide attempt and over two-thirds (N=90 [69%]) reported suicidal ideation. Patients who had attempted suicide demonstrated significantly higher median levels of DHEA and estradiol (Table 1). No significant differences were observed in androstenedione or testosterone levels. Suicidal ideation was not statistically associated with neuroactive steroid levels in this sample.
Younger age was associated with attempted suicide (z=–2.43, p=0.02, two-tailed Wilcoxon rank sum test) and suicidal ideation (z=2.29, p=0.02, two-tailed Wilcoxon rank sum test). The median age of patients with recent suicide attempts was 49.7 years (interquartile range=6.3), compared with 50.7 years (interquartile range=4.1) for patients with no suicide attempts. DHEA levels remained associated with attempted suicide in the logistic regression analyses controlling for age (odds ratio=1.05 for each 1 ng/ml increase in DHEA level, 95% confidence interval=0.99–1.10, p<0.06). Higher estradiol levels were no longer associated with a greater risk of recent suicide attempt after adjustment for age (p=0.31). No other selected variables were associated with a history of suicide attempt, including concurrent alcohol use disorder (χ2=1.06, df=1, p=0.32), current tobacco use (χ2=0.66, df=1, p=0.53), or childhood trauma (χ2=0.004, df=1, p=1.00).
Discussion
To our knowledge, this is the first report associating serum DHEA levels with suicide attempts in patients with PTSD. Since higher DHEA levels were correlated with a recent suicide attempt, this neuroactive steroid may be linked to the identification of suicide risk in patients with PTSD. Relevant to this finding, elevated DHEA levels have been reported in Israeli combat veterans with PTSD (7), and higher levels of DHEA sulfate have been observed in resettled refugees with PTSD in Sweden (8). Our findings of high suicidality rates in patients with PTSD are also consistent with the existing literature (1–3). In addition, a potential role for DHEA in depression has been suggested, although data are conflicting (20). Since this study was designed to investigate behavioral risks in a large group of mentally ill veterans, we did not assess depressive symptoms specifically. Therefore, the relationship among depression, suicidality, and DHEA cannot be determined in this initial study.
The clinical relevance of DHEA elevations in PTSD patients with a recent suicide attempt is currently unclear. Since DHEA has antiglucocorticoid (12, 13), neuroprotective (21, 22), and neurotrophic (23) effects, enhances learning and memory in animal models (24), and also increases neurogenesis in rodents (25) and human neural stem cell cultures (26), it is tempting to speculate that DHEA elevations may be compensatory in nature. Alternatively, DHEA negative modulation of GABAA receptors (15, 16) could theoretically exacerbate anxiety symptoms. In addition, DHEA activity at N-methyl-d-aspartic acid (23) and sigma-1 (27) receptors may also be relevant. Of note, a recent study (28) demonstrated that the antipsychotic clozapine lowers cerebral cortical DHEA levels in rodents. Since clozapine treatment reduces suicide attempts in patients with schizophrenia (29), it is possible that clozapine-induced decreases in DHEA (if these also occur in humans) may contribute to a reduction in suicidality following clozapine. The mechanisms mediating the association between higher DHEA levels and suicidality in PTSD will require further study.
Preliminary findings linking elevated DHEA levels in patients with PTSD to a suicide attempt in the past 6 months raises the possibility that DHEA is involved in PTSD pathophysiology. A relatively large number of subjects and a racially and ethnically diverse group are strengths of this study, but results should be interpreted with caution, given its cross-sectional design. Larger prospective efforts will be required to investigate this association more extensively.
Footnote
Presented at the 156th Annual Meeting of the American Psychiatric Association, San Francisco, May 17–22, 2003. Received June 20, 2003; revision received April 16, 2004; accepted May 26, 2004. From the Department of Psychiatry and Behavioral Sciences, Duke University Medical Center and Durham Veterans Affairs Medical Center. Address correspondence and reprint requests to Dr. Marx, Duke University Medical Center and Durham VA Medical Center, 508 Fulton St., Mental Health Service Line 116A, Durham, NC 27705; [email protected] (e-mail). Supported by a VA Research Career Development Award (Dr. Butterfield) and NIMH grant 1K23 MH-65080 (Dr. Marx).
References
1.
Kessler RC, Borges G, Walters EE: Prevalence of and risk factors for lifetime suicide attempts in the National Comorbidity Survey. Arch Gen Psychiatry 1999; 56:617–626
2.
Ferrada-Noli M, Asberg M, Ormstad K, Lundin T, Sundbom E: Suicidal behavior after severe trauma, part 1: PTSD diagnoses, psychiatric comorbidity, and assessments of suicidal behavior. J Trauma Stress 1998; 11:103–112
3.
Freeman TW, Roca V, Moore WM: A comparison of chronic combat-related (PTSD) patients with and without a history of suicide attempt. J Nerv Ment Dis 2000; 188:460–463
4.
Oquendo MA, Friend JM, Halberstam B, Brodsky BS, Burke AK, Grunebaum MF, Malone KM, Mann JJ: Association of comorbid posttraumatic stress disorder and major depression with greater risk for suicidal behavior. Am J Psychiatry 2003; 160:580–582
5.
Yehuda R: Current status of cortisol findings in post-traumatic stress disorder. Psychiatr Clin North Am 2002; 25:341–368
6.
Oquendo MA, Echavarria G, Galfalvy HC, Grunebaum MF, Burke A, Barrera A, Cooper TB, Malone KM, John Mann J: Lower cortisol levels in depressed patients with comorbid post-traumatic stress disorder. Neuropsychopharmacology 2003; 28:591–598
7.
Spivak B, Maayan R, Kotler M, Mester R, Gil-Ad I, Shtaif B, Weizman A: Elevated circulatory level of GABAA–antagonistic neurosteroids in patients with combat-related post-traumatic stress disorder. Psychol Med 2000; 30:1227–1231
8.
Sondergaard HP, Hansson LO, Theorell T: Elevated blood levels of dehydroepiandrosterone sulfate vary with symptom load in PTSD: findings from a longitudinal study of refugees in Sweden. Psychother Psychosom 2002; 71:298–303
9.
Paul SM, Purdy RH: Neuroactive steroids. FASEB J 1992; 6:2311–2322
10.
Wolkowitz OM, Epel ES, Reus VI: Stress hormone-related psychopathology: pathophysiological and treatment implications. World J Biol Psychiatry 2001; 2:115–143
11.
Morrow AL, Devaud LL, Purdy RH, Paul SM: Neuroactive steroid modulators of the stress response. Ann NY Acad Sci 1995; 771:257–272
12.
Kimonides VG, Spillantini MG, Sofroniew MV, Fawcett JW, Herbert J: Dehydroepiandrosterone antagonizes the neurotoxic effects of corticosterone and translocation of stress-activated protein kinase 3 in hippocampal primary cultures. Neuroscience 1999; 89:429–436
13.
Kalimi M, Shafagoj Y, Loria R, Padgett D, Regelson W: Anti-glucocorticoid effects of dehydroepiandrosterone (DHEA). Mol Cell Biochem 1994; 131:99–104
14.
Melchior CL, Ritzmann RF: Dehydroepiandrosterone is anxiolytic in mice on the plus maze. Pharmacol Biochem Behav 1994; 47:437–441
15.
Park-Chung M, Malayev A, Purdy RH, Gibbs TT, Farb DH: Sulfated and unsulfated steroids modulate γ-aminobutyric acidA receptor function through distinct sites. Brain Res 1999; 830:72–87
16.
Imamura M, Prasad C: Modulation of GABA-gated chloride ion influx in the brain by dehydroepiandrosterone and its metabolites. Biochem Biophys Res Commun 1998; 243:771–775
17.
Genazzani AD, Stomati M, Bernardi F, Pieri M, Rovati L, Genazzani AR: Long-term low-dose dehydroepiandrosterone oral supplementation in early and late postmenopausal women modulates endocrine parameters and synthesis of neuroactive steroids. Fertil Steril 2003; 80:1495–1501
18.
Blanchard EB, Jones-Alexander J, Buckley TC, Forneris CA: Psychometric properties of the PTSD Checklist (PCL). Behav Res Ther 1996; 34:669–673
19.
Rosenberg SD, Goodman LA, Osher FC, Swartz MS, Essock SM, Butterfield MI, Constantine NT, Wolford GL, Salyers MP: Prevalence of HIV, hepatitis B, and hepatitis C in people with severe mental illness. Am J Public Health 2001; 91:31–37
20.
Van Broekhoven F, Verkes RJ: Neurosteroids in depression: a review. Psychopharmacology (Berl) 2003; 165:97–110
21.
Kimonides VG, Khatibi NH, Svendsen CN, Sofroniew MV, Herbert J: Dehydroepiandrosterone (DHEA) and DHEA-sulfate (DHEAS) protect hippocampal neurons against excitatory amino acid-induced neurotoxicity. Proc Natl Acad Sci USA 1998; 95:1852–1857
22.
Marx CE, Jarskog LF, Lauder JM, Gilmore JH, Lieberman JA, Morrow AL: Neurosteroid modulation of embryonic neuronal survival in vitro following anoxia. Brain Res 2000; 871:104–112
23.
Compagnone NA, Mellon SH: Dehydroepiandrosterone: a potential signaling molecule for neocortical organization during development. Proc Natl Acad Sci USA 1998; 95:4678–4683
24.
Vallee M, Mayo W, Le Moal M: Role of pregnenolone, dehydroepiandrosterone and their sulfate esters on learning and memory in cognitive aging. Brain Res Brain Res Rev 2001; 37:301–312
25.
Karishma KK, Herbert J: Dehydroepiandrosterone (DHEA) stimulates neurogenesis in the hippocampus of the rat, promotes survival of newly formed neurons and prevents corticosterone-induced suppression. Eur J Neurosci 2002; 16:445–453
26.
Suzuki M, Wright LS, Marwah P, Lardy HA, Svendsen CN: Mitotic and neurogenic effects of dehydroepiandrosterone (DHEA) on human neural stem cell cultures derived from the fetal cortex. Proc Natl Acad Sci USA 2004; 101:3202–3207
27.
Maurice T, Urani A, Phan VL, Romieu P: The interaction between neuroactive steroids and the sigma1 receptor function: behavioral consequences and therapeutic opportunities. Brain Res Brain Res Rev 2001; 37:116–132
28.
Nechmad A, Maayan R, Ramadan E, Morad O, Poyurovsky M, Weizman A: Clozapine decreases rat brain dehydroepiandrosterone and dehydroepiandrosterone sulfate levels. Eur Neuropsychopharmacol 2003; 13:29–31
29.
Meltzer HY, Alphs L, Green AI, Altamura AC, Anand R, Bertoldi A, Bourgeois M, Chouinard G, Islam MZ, Kane J, Krishnan R, Lindenmayer JP, Potkin S (International Suicide Prevention Trial Study Group): Clozapine treatment for suicidality in schizophrenia: International Suicide Prevention Trial (InterSePT). Arch Gen Psychiatry 2003; 60:82–91
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