Inferior Frontal White Matter Anisotropy and Negative Symptoms of Schizophrenia: A Diffusion Tensor Imaging Study

After complete description of the study to the subjects, written informed consent was obtained from 10 male schizophrenic in- and outpatients. The patients were all under 50 years of age and met the DSM-IV criteria for schizophrenia. Patients with depression meeting diagnostic criteria, drug abuse/dependence within the last year, or history of neurological disease were excluded. The subjects were selected on the basis of a priori clinical familiarity in order to obtain a range of negative symptom severity. All subjects were receiving antipsychotic medication. The clinical rating measures included the Brief Psychiatric Rating Scale (BPRS) (14) and the Schedule for Assessment of Negative Symptoms (SANS) (15). Ratings were obtained when the patients were considered to be at maximal clinical stability (“baseline”), up to 40 days after the MRI scan. For the statistical analyses, negative symptom ratings were reduced to the SANS subscale global scores, a single total of the SANS global subscores, and the score on the withdrawal-retardation factor from the BPRS.

To minimize artifacts induced by eddy currents, the diffusion tensor imaging data were acquired by using a double-echo pulsed-gradient echo planar imaging method without cardiac gating, with TR=6 sec, TE=100 msec, field of view=24 cm, 128×128 matrix reconstructed to 256×256, b=1000 sec/mm², four averages, six noncollinear directions aligned to the anterior-posterior commissure (AC-PC) plane, and acquisition time=2 minutes, 36 seconds. Fractional anisotropy and mean diffusivity were computed as described in a previous report (16). All raw and processed images were examined for artifacts by a reviewer who was blind to subject identity (K.O.L. or S.J.C.).

Standardized circular regions of interest were positioned, by an investigator blind to subject identity (S.J.C.), on the T₂-weighted image (b=0) of the diffusion tensor imaging data set; thus, coregistration of images across different MRI acquisition methods was not needed. A single region of interest was placed bilaterally in the frontal white matter of five consecutive 5-mm-thick slices. These slices included three that were above the index slice, the index slice itself (defined as the most inferior slice containing the genu of the corpus callosum—approximately 10 mm above the AC-PC plane), and one slice below. For the three most superior frontal slices, large regions of interest (area=84.4 mm²) were centered in white matter with the posterior boundary of each region of interest on the imaginary line formed across the anterior most tips of the two frontal horns of the lateral ventricles. For the two most inferior frontal slices, medium-sized regions of interest (area=43.5 mm²) were centered in white matter with the posterior boundary of the region of interest on the imaginary line linking the most lateral extent of the sylvian fissures across the hemispheres.

Spearman’s rank order correlation coefficients were calculated to assess the relationship of negative symptom variables with white matter fractional anisotropy and mean diffusivity in the five frontal regions. To reduce the number of comparisons, we averaged fractional anisotropy and mean diffusivity across hemispheres to yield one fractional anisotropy and mean diffusivity value per slice. For these analyses, alpha was set at 0.01, two-tailed. The reported results represent the only hypothesis regarding anatomic regions and symptom scores that was tested.

The mean age of the 10 patients was 41 years (SD=9); their mean duration of illness was 17 years (SD=9), and their mean sum of SANS global subscores was 12.8 (range=9–18).

Across subjects, frontal white matter fractional anisotropy at 5 mm below the AC-PC plane was inversely correlated with the severity of affective blunting (r=–0.72, p=0.02) and anhedonia (r=–0.69, p=0.03), according to the SANS subscales, and with a higher overall sum of SANS global subscores (r=–0.84, p=0.002) (Figure 1). Similarly, greater withdrawal-retardation, indicated by the BPRS, was inversely correlated with white matter fractional anisotropy in the same inferior frontal region (r=–0.76, p=0.02).

The main finding of this study is that fractional anisotropy in the inferior frontal white matter is lower among patients with more severe negative symptoms than among other patients with schizophrenia. This finding is consistent with those of our previous structural MRI study (3), in which significant inverse correlations were found between the severity of negative symptoms and prefrontal white matter volumes. These findings suggest that orbitofrontal white matter microstructure may play an important role in the pathophysiology of negative symptoms in schizophrenia by disruption of cortical-cortical or cortical-subcortical connectivity within neuronal networks. Further elucidation of such abnormalities may shed light on the “anatomy” of negative symptoms. Given the limitations of this preliminary study, these results also need to be replicated with a larger number of subjects.

Received March 18, 2002; revision received Sept. 3, 2002; accepted Sept. 6, 2002. From the Mental Health Service, VA New York Harbor Healthcare System; and the Nathan S. Kline Institute for Psychiatric Research, Orangeburg, N.Y. Address reprint requests to Dr. Wolkin, Mental Health Service (11M), VA New York Harbor Healthcare System, 423 East 23rd St., New York, NY 10010; [email protected] (e-mail). Supported by the Department of Veterans Affairs and by NIMH grant MH-60662.